??【產(chǎn)品詳細(xì)介紹】
Biotium貨號(hào)41003的GelRed®核酸凝膠染料（10,000×水溶液）是專為瓊脂糖/聚丙烯酰胺凝膠電泳設(shè)計(jì)的革命性熒光染料。作為溴化乙錠（EB）的安全替代品，它通過分子結(jié)構(gòu)實(shí)現(xiàn)DNA的高效結(jié)合，在302nm紫外透射儀或藍(lán)光切膠儀下可激發(fā)出鮮亮紅色熒光。產(chǎn)品規(guī)格為1mL/瓶，適用于常規(guī)PCR產(chǎn)物、質(zhì)粒DNA及RNA的凝膠分析，兼容預(yù)制膠與后染色兩種使用方式，特別優(yōu)化了低濃度樣品（0.5-5ng/band）的檢測(cè)靈敏度。
??【核心優(yōu)勢(shì)】
1?? 安全認(rèn)證：通過美國(guó)NIOSH突變性檢測(cè)，細(xì)胞毒性比EB低1000倍，實(shí)驗(yàn)室操作無(wú)需特殊防護(hù)
2?? 超敏檢測(cè)：可檢測(cè)0.5ng DNA條帶，靈敏度是SYBR® Safe的2倍（實(shí)驗(yàn)數(shù)據(jù)見Biotium#41003）
3?? 快速顯影：室溫染色15分鐘即可成像，比傳統(tǒng)EB染色縮短60%時(shí)間
4?? 多波長(zhǎng)兼容：支持254-365nm UV及470nm藍(lán)光雙模式激發(fā)，適配多數(shù)凝膠成像系統(tǒng)
5?? 實(shí)驗(yàn)友好：水溶性配方無(wú)需有機(jī)溶劑，染色后凝膠可長(zhǎng)期保存不淬滅
6?? 文獻(xiàn)背書：被Nature Methods等頂刊引用超2000次，全球500+實(shí)驗(yàn)室長(zhǎng)期使用
??【常見疑問解答】
Q1：是否需要更換現(xiàn)有成像設(shè)備？
A：兼容！GelRed®支持254nm/302nm/365nm UV及470nm藍(lán)光四檔激發(fā)，無(wú)需額外投資設(shè)備
Q2：操作時(shí)是否需要戴手套？
A：建議佩戴！雖通過安全認(rèn)證，但作為良好實(shí)驗(yàn)室規(guī)范（GLP），接觸染料時(shí)仍建議佩戴一次性手套
Q3：能否用于RNA檢測(cè)？
A：經(jīng)驗(yàn)證！在6%變性聚丙烯酰胺凝膠中可清晰顯示50ng RNA條帶（附應(yīng)用指南文檔）
Q4：價(jià)格比EB貴很多？
A：算筆賬！單次使用成本僅￥0.8-1.5元，且節(jié)省防護(hù)設(shè)備與廢液處理費(fèi)用，長(zhǎng)期使用更經(jīng)濟(jì)
Q5：開封后保質(zhì)期多久？
A：避光4℃保存可達(dá)12個(gè)月，我們提供免費(fèi)分裝瓶（聯(lián)系客服獲取#41003-SP配件）
Q6：是否影響下游實(shí)驗(yàn)？
A：已驗(yàn)證！染色后DNA可直接用于克隆、測(cè)序等操作，回收效率達(dá)85%以上（第三方檢測(cè)報(bào)告見）
Q7：能否用于變性梯度凝膠？
A：特殊配方！在65℃高溫下仍保持穩(wěn)定，特別適合DGGE等特殊電泳應(yīng)用
Q8：新手如何優(yōu)化染色效果？
A：下載《GelRed®染色手冊(cè)》！含5種凝膠濃度推薦方案及常見問題視頻教程
（注：本文內(nèi)容基于品牌公開資料及行業(yè)常規(guī)信息整理，具體以品牌信息文檔為準(zhǔn)。）
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貨號(hào) | 品名 | 規(guī)格 | 品牌 |
E90002 | PMA-Lite™ LED Photolysis Device | 1EA | Biotium |
90066-1 | MTSEA Biotin-XX | 50mg | Biotium |
41029-50G | GelRed®AgaroseLE | 50G | Biotium |
41010-1mL | 6X GelRed Prestain Buffer with Orange Tracking Dye | 1mL | Biotium |
40060-T | RedDot™1 Far-Red Nuclear Stain, 200X in Water | 25uL | Biotium |
40027-T | dCTP CF® Dye Conjugates | 5 nmol | Biotium |
40020-1 | 5-Aminoallyl-dUTP (AA-dUTP), Lyophilized Powder | 1 mg | Biotium |
40002-T | dUTP CF® Dye Conjugates | 5 nmol | Biotium |
33022-T | GloMelt™ Thermal Shift Protein Stability kit | 1kit | Biotium |
33022-1 | GloMelt™ Thermal Shift Protein Stability kit | 1kit | Biotium |
33021-T | GloMelt™ Thermal Shift Protein Stability kit | 1kit | Biotium |
33021-1 | GloMelt™ Thermal Shift Protein Stability kit | 1kit | Biotium |
31077-T | EvaGreen®Plus,20XinWater,1mL | 1ML | Biotium |
31069-T | AccuGreen™ Broad Range dsDNA Quantitation Kit (100 assays) | 1kit | Biotium |
31041-1 | Forget-Me-Not™ EvaGreen® qPCR Master Mix (500 reactions) | 5x1ml | Biotium |
31028-T | AccuClear® Ultra High Sensitivity dsDNA Quantitation Kit with DNA Standard, trial size (200 assays) | 1kit | Biotium |
31003-1 | FastEvaGreen®MasterMixforqPCR(500rxn) | 5x1ML | Biotium |
31000-T | EvaGreen® Dye, 20X in Water | 1mL | Biotium |
30114-T | ExoBrite™ 640/660 EV Membrane Staining Kit, 100 labelings | 1KIT | Biotium |
30096-T | MemBrite™ Fix 594/615 Cell Surface Staining Kit, 100 Reactions | 1KIT | Biotium |
30090-T | CellBrite™ Fix 488 Membrane Labeling Kit, Trial Size | 1KIT | Biotium |
30089-T | CellBrite™ Fix 640 Membrane Labeling Kit, Trial Size | 1KIT | Biotium |
30029-T | NUCVIEW 488 CASPASE 3 ASSAY KIT, TRIAL SIZE 1ST | 1ST | Biotium |
30028-L3 | Steady-Luc™ Firefly HTS Assay Kit (Lyophilized) 10 x 1000 Assays | 10000TST | Biotium |
30020-T | ATP-Glo™ Bioluminometric Cell Viability Assay Kit (50 assays) | 1KIT | Biotium |
29092-10mg | Allophycocyanin (APC), cross-linked lyophilized solid, ammonium sulfate free | 10mg | Biotium |
29091-10mg | R-Phycoerythrin (RPE), lyophilized solid, ammonium sulfate free | 10mg | Biotium |
29028-1 | WheatGermAgglutinin,CF®405MConjugate | 1mg | Biotium |
29027-1 | CF®405S WGA CF®405S 404/431 nm | 1mg | Biotium |
29026-1 | CF®640R WGA | 1mg | Biotium |
29023-1 | WheatGermAgglutinin,CF®594Conjugate | 1mg | Biotium |
29022-1 | WheatGermAgglutinin,CF®488AConjugate | 1mg | Biotium |
23014-T | TrueBlack® Plus Lipofuscin Autofluorescence Quencher, 40X in DMSO | 50ul | Biotium |
23005-1 | CoverGrip™ Coverslip Sealant Refill | 100ml | Biotium |
21005-1L | One-Step Blue® Protein Gel Stain | 1L | Biotium |
21004-1L | One-Step Lumitein™ Protein Gel Stain, 1X | 1L | Biotium |
21003-1L | One-Step Blue Protein Gel Stain, 1X | 1L | Biotium |
20813-50uL | Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, CF® Dye Conjugates, Single Label for STORM | 50uL | Biotium |
20104-1 | CF®568 Goat Anti-Chicken IgY (IgG) (H+L), Highly Cross-Adsorbed, 2 mg/mL | 50ul | Biotium |
20074-1mg | CF488A 驢 抗 人 IgG (H+L), highly cross-adsorbed | 1mg | Biotium |
20071-1 | CF®488A Rabbit Anti-Human IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL | 50ul | Biotium |
20022-1mg | CF®488A Goat Anti-Human IgG (H+L), Highly Cross-Adsorbed | 1mg | Biotium |
10125-50uG | Coelenterazine 400A (DEEPBLUEC, A TRADEM | 50uG | Biotium |
10111-50uG | Coelenterazine H | 50uG | Biotium |
10110-50uG | Coelenterazine (ALSO KNOWN AS NATIVE COE | 50uG | Biotium |
00044-T | phalloidin, CF568 | 50U | Biotium |
00042-T | Phalloidin, CF®488A | 50U | Biotium |
00034-T | Phalloidin,CF®405M | 50U | Biotium |
00010-1 | Alpha-Bungarotoxin | 1MG | Biotium |
00005-100ug | α-Bungarotoxin, CF® Dye and Other Conjugates | 100ug | Biotium |
#70037 | SCAS | 100 mg | Biotium |
92120 | CF®488A,succinimidylester | 1UMOL | Biotium |
91020 | MTSES (2-Sulfonatoethyl methanethiosulfonate sodium salt) is a negatively charged MTS reagent. | 100mg | Biotium |
90066 | Biotin-XX MTSEA | 10mg | Biotium |
80112 | CF®555-Dextran10,000MW,AnionicandFixable | 1MG | Biotium |
80110 | CF®488A-Dextran 10,000 MW, Anionic and Fixable | 1MG | Biotium |
80014 | Hydroxystilbamidine (Fluoro-Gold™) | 10mg | Biotium |
41005 | GelGreen® Nucleic Acid Gel Stain, 10,000X in Water | 0.5ML | Biotium |
41004 | GelGreen® 10,000X in DMSO | 0.5 mL | Biotium |
41003 | GelRed® Nucleic Acid Gel Stain | 0.5ml | Biotium |
41002 | GelRed® Nucleic Acid Gel Stain 10.000X in DMSO | 0.5ML | Biotium |
41001 | GelRed® Nucleic Acid Gel Stain, 3X in Water | 4L | Biotium |
40069 | PMAxx™ Dye, 20 mM in H2O | 100ul | Biotium |
40061 | RedDot™2 Far-Red Nuclear Stain, 200X in DMSO | 250ul | Biotium |
40050 | Ethidium homodimer III | 1mg | Biotium |
40035 | Biotin-11-CTP, 10 mM Solution | 25uL | Biotium |
40027 | dCTP CF® Dye Conjugates | 25 nmol | Biotium |
40021 | 5-Aminoallyl-UTP, Sodium Salt, 10 mM in TE Buffer | 100ul | Biotium |
40019 | PMA (Propidium Monoazide) | 100uL | Biotium |
40013 | PMA (Propidium Monoazide) | 1mg | Biotium |
40012 | DMAO, 2mM in DMSO | 1ml | Biotium |
40008 | CF®488A-dUTP,LyophilizedPowder | 25nmol | Biotium |
40002 | dUTP CF® Dye Conjugates | 25 nmol | Biotium |
31077 | EvaGreen® Plus, 20X in Water, 5x1 mL | 5ml | Biotium |
31075 | Viability PCR Starter Kit with PMA | 1kit | Biotium |
31038 | PMA Enhancer for Gram Negative Bacteria, 5X Solution | 16 mL | Biotium |
31019 | EvaGreen®, 2000X in DMSO | 50uL | Biotium |
31000 | EvaGreen®, 20X in Water, 5x1 mL | 5x1ml | Biotium |
30090 | CellBrite™ Fix 488 Membrane Labeling Kit | 1KIT | Biotium |
30089 | CellBrite™ Fix 640 Membrane Labeling Kit | 1KIT | Biotium |
30072 | NucView® 488 and RedDot™ 2 Apoptosis & Necrosis Kit (100 assays) | 1KIT | Biotium |
30029 | NucView® 488 Caspase 3 Assay Kit | 1KIT | Biotium |
30024 | CellBrite™ Blue Cytoplasmic Membrane-Labeling Kit | 1KIT | Biotium |
30023 | CellBrite™RedCytoplasmicMembraneDye | 1ML | Biotium |
29052 | ROX Passive Reference Dye | 5ml | Biotium |
29051 | EvaEZ™ Fluorometric Polymerase Activity Assay Kit | 2 x 1 mL | Biotium |
29050 | Cheetah™ Hot Start Taq DNA Polymerase | 500EU | Biotium |
29022 | WheatGermAgglutinin,CF®488AConjugate | 5x1mg | Biotium |
23012 | TrueBlack® IF Background Suppressor System (Permeabilizing) | 200 assays | Biotium |
23007 | TrueBlack® Lipofuscin Autofluorescence Quencher, 20X in DMF | 1mL | Biotium |
23005 | CoverGrip™CoverslipSealant | 15mL | Biotium |
23001 | EverBrite™ Mounting Medium | 1ml | Biotium |
22010 | 10XFishGelatinBlockingAgent | 100ML | Biotium |
22003 | Mini Cell Scrapers | 200ST | Biotium |
20432 | CF®488A Rabbit Anti-FLAG | 100 uL | Biotium |
20302 | Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed (Min X Rat) | 0.5mL | Biotium |
20282 | CF®647 Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL | 500uL | Biotium |
20092 | Donkey Anti-Rat IgG (H+L), Highly Cross-Adsorbed | 0.5mL | Biotium |
20015 | CF®488ADonkeyAnti-RabbitIgG(H+L),HighlyCross-Adsorbed,2mg/mL | 500uL | Biotium |
10030 | FDP (high purity) | 5 mg | Biotium |
